ABSTRACT
The objective this study was to determine the effect of phytohemagglutinin (PHA) on survival, growth and gene expression in caprine secondary follicles cultured in vitro. Secondary follicles (∼0.2 mm) were isolated from the cortex of caprine ovaries and cultured individually for 6 days in α-MEM+ supplemented with PHA (0, 1, 10, 50, 100, or 200 µg/mL). After 6 days of culture, follicle diameter and survival, antrum formation, ultrastructure and expression of mRNA for FSH receptors (FSH-R), proliferating cell nuclear antigen (PCNA), and neuronal nitric oxide synthase were determined. All treatments maintained follicular survival [α-MEM+ (94.59%); 1 µg/mL PHA (96.43%); 10 µg/mL PHA (84.85%); 50 µg/mL PHA (85.29%); 100 µg/mL PHA (88.57%), and 200 µg/mL PHA (87.50)], but the presence of 10 µg/mL PHA in the culture medium increased the antrum formation rate (21.21%) when compared with control (5.41%, P < 0.05) and ensured the maintenance of oocyte and granulosa cell ultrastructures after 6 days of culture. The expression of mRNA for FSH-R (2.7 ± 0.1) and PCNA (4.4 ± 0.2) was also significantly increased in follicles cultured with 10 µg/mL PHA in relation to those cultured in α-MEM+ (1.0 ± 0.1). In conclusion, supplementation of culture medium with 10 µg/mL PHA maintains the follicular viability and ultrastructure, and promotes the formation of antral cavity after 6 days of culture in vitro.
Subject(s)
Animals , Female , Follicle Stimulating Hormone/metabolism , Mitogens/pharmacology , Ovarian Follicle/drug effects , Phytohemagglutinins/pharmacology , Follicle Stimulating Hormone/genetics , Goats , In Vitro Techniques , Ovarian Follicle/growth & development , Ovarian Follicle/ultrastructure , Proliferating Cell Nuclear Antigen/metabolism , RNA, Messenger/metabolismABSTRACT
Pacientes com HIV e DPOC são mais facilmente acometidos pela colonização fúngica intracavitária pulmonar, a qual gera uma fibrose pericavitária, bem como epitelização endocavitária oriunda das comunicações brônquicas. Relata-se o caso de um paciente com DPOC o qual foi diagnosticado bola fúngica através de Tomografia de tórax. Através da fibrobroncoscopia, verificou- se a presença de leveduras com características morfológicas de Paracoccidióides brasiliensis.
Patients with HIV and COPD are more easily affected by fungal colonization in the lung cavity which generates a around the cavity fibrosis and epithelization internal cavities derives from the bronchi. It?s reported the case of a patient with COPD who was diagnosed fungal ball through tomography of the chest. The bronchoscopy there was the presence of yeast with morphological characteristics of Paracoccidioides brasiliensis.